Infectious Diseases >> Application >> African Trypanosomiasis
Prepared in collaboration with Noriyuki Murakami
Sleeping sickness, also known as Human African Trypanosomiasis (HAT), is caused by Trypanosoma brucei rhodensiense in Eastern Africa and Trypanosoma brucei gambiense in Western Africa . Both protozoan species are morphologically indistinguishable, but have drastically different epidemiological features. Several species of hematophagous glossina, commonly known as tsetse flies, are the vectors of these related diseases, and are responsible for cyclical transmission of the parasitic protozoan between numerous vertebrate hosts. Both forms of sleeping sickness affect the central nervous system. The term “sleeping sickness” is derived from the West African form of trypanosomiasis, primarily because invasion of the cerebrospinal fluid and brain after infection of the blood is often delayed, resulting in symptoms of extreme fatigue that can last for several years before the severe phase of the disease sets in; toxemia, coma and death. In contrast, the typical East African form of trypanosomiasis is characterized by rapid and acute development of the disease, and untreated patients can die within weeks or months of infection.
Although epidemics of sleeping sickness were more rampant in the past, the most recent WHO estimates put 60 million people at risk of HAT today with approximately 500,000 people currently with infections. The disease is discontinuously spread over 9 million square kilometers and affects populations across 37 sub-Saharan countries. Animal trypanosomiasis, caused by a wider number of trypanosome species and carried with higher prevalence by a greater number of glossina species, is invariably the greater epidemic across the African continent with dire economic consequences. In general, trypanosome infections that threaten livestock are over 100 to 150-fold higher in G. morsitans than the trypanosome infections that cause human trypanosomiasis (Jordan 1976). Historically, the impact of animal trypanosomiasis were so profound that it influenced the migration routes of cattle-owning tribes into the continent who were forced to avoid the G. morsitans “fly-belts” (Ford 1960), as well as the movements of early European and Arab settlers into the continent who depended on horses and oxen (McKelvey 1973).
Of the 31 species of glossina in the African continent, eleven are important for transmitting the infection to humans. Today, most efforts to reduce transmission of the disease to humans and other vertebrate reservoirs focus on the control of only these species. Clinical treatment of both early and late onset sleeping sickness is limited and far from up-to-date, and thus cannot be relied upon for controlling the spread of the infection during times of epidemics. Therefore, understanding the ecological factors that determine patterns of transmission to people, and those that play a role in the re-emergence of the disease is vital to the design of new effective programs to reduce the burden of disease in human populations. This web site emphasizes the ecology of the tsetse vectors and also documents some of the more useful control strategies to limit their populations.
The earliest recorded account of sleeping sickness comes from upper Niger during the 14th century in the historical writings of Ibn Khaldoun, who wrote about the disease in his account of the history of North Africa . The next report came from Guinea in 1734 (Atkins, 1978). In 1803, the diseases that caused visible swollen lymph glands in West Africa came to be known as Winterbottom's sign, after the description of the disease by Winterbottom. Such signs were readily recognized by slave traders who avoided trading and buying slaves who displayed those symptoms.
The earliest detection of trypanosomes in human blood was in 1902, when R.M. Forde discovered what was then thought to be filiaria in the blood of a steamboat captain who had traveled extensively along the River Gambia. Similar discoveries of filiaria-like organisms in the blood were made by J.H. Cook in East Africa, but confusion arose as to how filiaria worms could cause such varying clinical symptoms. It was J.E. Dutton who, during a visit to Gambia, first correctly identified the parasite as a trypanosome and subsequently named it Trypanosoma gambiense . In 1902, A. Castellani observed the presence of trypanosomes in cerebrospinal fluid taken from a sleeping sickness patient, but it wasn't until 1903 that D. Bruce correctly recognized that trypanosomes were the causative agents of sleeping sickness transmitted to humans by tsetse flies, and that “trypanosome fever” and “sleeping sickness” - both thought to be different diseases at the time - were in fact the same.
Morphologically indistinguishable from the West African species as well as the animal infecting species Trypanosoma brucei brucei, Trypanosoma brucei rhodensiense was first discovered in Zambia by J.W.W. Stephens and H.B. Fantham in 1910. By 1926, T.b. rhodensiense could be found along the fly-belt between Tabora and Kigoma, Tanzania . The difficulties in identifying this virulent form of sleeping sickness lead to uncertainties today regarding the evolution and progression of T.b. rhodensiense through the continent, although it is generally agreed upon that it originated from the West African form.
The earliest recorded major epidemics of sleeping sickness took place in Uganda and Congo between 1896 and 1908, where roughly 500,000 people were estimated to have died in the Congo Basin, and approximately 300,000 died in Busoga, Uganda . With the Rift Valley transecting the country, Uganda is in the precarious position of having foci of both forms of diseases which resulted in two other major epidemics of sleeping sickness - one in the late 1940's and another in 1980. Throughout West Africa, smaller epidemics of sleeping sickness rapidly spread from Senegal to Cameroon during the 1920's, and died down by the late 1940's.
African trypanosomes are extracellular organisms, both in the mammalian and insect host. T. b. gambiense and T. b. rhodesiense are morphologically indistinguishable, measuring 25-40 µ m in length. Infection in the human host begins when the infective stage, known as the metacyclic stage, is injected intradermally by the tsetse fly. The organisms rapidly transform into blood-stage trypomastigotes (long, slender forms), and divide by binary fission in the interstitial spaces at the site of the bite wound. The buildup of metabolic wastes and cell debris leads to the formation of a chancre .
Trypanosomes have a single specialized mitochondrion called a kinetoplast mitochondrion. One of its unusual features is that all of the DNA of the mitochondrion, which can be up to 25% of the total cell DNA, is localized in the kinetoplast, adjacent to the flagellar pocket. Kinetoplast DNA or kDNA exists in two forms: mini-circles and maxi-circles. Mini-circle DNA encodes guide RNAs that direct extensive editing of RNA transcripts post-transcriptionally. Maxi-circle DNA contains sequences that, when edited, direct translation of typically mitochondrially-encoded proteins.
In the vertebrate host, trypanosomes depend entirely upon glucose for energy and are highly aerobic, despite the fact that the kinetoplast-mitochondrion completely lacks cytochromes. Instead, mitochondrial oxygen consumption is based on an alternative oxidase that does not produce ATP. When in the insect vector, the parasite develops a conventional cytochrome chain and TCA cycle.
The surface of the trypanosome has numerous membrane-associated transport proteins for obtaining nucleic acid bases, glucose, and other small molecular weight nutrients. None of these proteins react well with antibodies, because although they lie in exposed regions of membrane, they are shielded by allosteric interference provided by the variant surface glycoprotein (VSG) coat proteins. This flagellated stage enters the bloodstream through the lymphatics and divides further, producing a patent parasitemia. The number of parasites in the blood is generally so low that diagnosis by microscopic examination is often negative. At some point, trypanosomes enter the central nervous system, with serious pathological consequences for humans. Some parasites transform into the non-dividing short, stumpy form , which has a biochemistry similar to those of the long, slender form and the form found in the insect vector.
The tsetse fly becomes infected by ingesting a blood meal from an infected host. These short, stumpy forms are pre-adapted to the vector, having a well developed mitochondrion with a partial TCA cycle. In the insect vector, the trypanosomes develop into procyclic trypomastigotes in the midgut of the fly, and continue to divide for approximately 10 days. Here they gain a fully functional cytochrome system and TCA cycle. When the division cycles are completed, the organisms migrate to the salivary glands, and transform into epimastigotes. These forms, in turn, divide and transform further into metacyclic trypanosomes, the infective stage for humans and reservoir hosts. The cycle in the insect takes 25-50 days, depending upon the species of the fly, the strain of the trypanosome, and the ambient temperature. If tsetse flies ingest more than one strain of trypanosome, there is the possibility of genetic exchange between the two strains, generating an increase in genetic diversity in an organism that may not have a sexual cycle.
Flies can remain infected for life (2-3 months). Tsetse flies inject over 40,000 metacyclic trypanosomes when they take a blood meal. The minimum infective dose for most hosts is 300-500 organisms, although experimental animals have been infected with a single organism. Infection can also be acquired by eating raw meat from an infected animal. In East Africa, this mode of transmission may be important in maintaining the cycle in some reservoir hosts.
There are 31 species and subspecies of tsetse flies under the genus Glossina, family Glossinidae, and order Diptera. Tsetse flies are largely classified into three subgenera based on morphological differences in the structure of the genitalia: Morsitans (Glossina), Palpalis (Nemorhina), and Fusca (Austenina) groups. Although the tsetse flies can be found over some 9 million squared kilometers of the African continent, presence of glossina populations throughout the continent are far from continuous. In general, the Sahara and Somali Deserts limit the populations in the north, extending across the entire continent from Senegal in the west to southern Somalia in the east. Tsetse populations are denser in West and Central Africa, and are found more sporadically to the East and down to the borders of the Kalahari and Namibian Deserts in Southern Africa . Although tsetse fly habitats may vary considerably, climate and altitude - through their direct effects on vegetation, rainfall, and temperature - are still the primary determinants for proliferation. Unlike other insects, there are no seasonal interruptions in the life cycles of tsetse flies. However both adult longevity and puparial duration are related to temperature, and a significant seasonal decline in tsetse populations is normal, particularly in savannah habitats during the dry season. The 3 groups of tsetse flies are generally adapted to different habitats and ecozones.
Brief summary of ecological zones in Africa (adapted from Jahnke, 1982)
There are seven species that fall into the morsitans group. All are potential vectors of both human and animal trypanosomiasis. The three Glossina morsitans subspecies are exceptionally good vectors of trypanosomes. All species within this group inhabit the savanna woodlands that surround the two major blocks of lowland rain forests in Africa . The distributions of tsetse flies in this group closely follow the distributions of wild animals and water sources. In the wetter areas the flies are observed to roam more widely over the woodland, but in drier areas their movements are restricted to mesophytic vegetation of the watercourses.
In Eastern and Southern Africa where Glossina morsitans morsitans is the primary vector for human and animal trypanosomiasis, the "miombo" woodlands (Brachystegia-Julbernardia) that extend from Mozambique to Tanzania, as well as the "mopane" woodlands (Colophospermum mopane) in Zambia and Zimbabwe are the typical habitats. The other subspecies Glossina morsitans centralis dominate northwards from Botswana and Angola into Southern Uganda, closer inland towards the lowland forests but also occurring in miombo vegetation. Glossina morsitans submorsitans have an east to west distribution from Ethiopia to Senegal in ‘doka' woodlands where the vegetation is dominated by Isoberlinia species, and can be sporadically found to occur in the southern Guinea savanna vegetation zone as well in the drier Sudan zone.
Glossina swynnertoni is restricted to a small area between northern Tanzania (Serengeti) and Southern Kenya (Masai Mara) where the Acacia-Commiphora vegetation can be found, along with an abundance of wild life. Glossina longipalpis and Glossina pallidipes both have a much wider range of possible habitats displaying versatility by existing in different vegetation types. Glossina longipalpis occurs mainly in the narrow savannah belt just north of the rain forest in West Africa, from Guinea to Cameroon . Much of the savannah is derived from human destruction of the climax forest vegetation, and as a result is spreading southwards. The highly mobile Glossina pallidipes occurs in East Africa from Mozambique to Ethiopia over a relatively wide range of climatic and vegetation conditions. Finally, Glossina austeni occupy secondary scrub, thicket and islands of forest along the East African coast from Mozambique to Somalia. However, its distribution is discontinuous, rarely being found at altitudes over 200 meters or more than 250 km inland from the coast.
Of the nine species in the palpalis subgenera, the five palpalis and fuscipes subspecies are vectors of both human and animal trypanosomiasis. Although flies in this group are continuously found in the lowland rainforests, some are known to extend out to the savannah regions particularly along rivers and streams. The habitat of the palpalis flies occur mainly in the drainage systems leading to the Atlantic or the Mediterranean Ocean, extending from the wet mangrove and rain forests along the coastal regions of West Africa to the drier savannah areas just north of the rain forests. The flies in the palpalis group are less tolerant to the wide range of climatic conditions of the savannah belt, and are therefore restricted to the ecoclimate of the watercourses from where they derive their label as the ‘riverine species'. Many of the palpalis species, such as the Glossina palpalis palpalis in Côte d'Ivoire, prefer peri-domestic conditions and have been observed to maintain close association with villages (Baldry, 1980). Similarly, it is thought that the advancement of Glossina tachinoides into Eastern Côte d'Ivoire and Togo have been attributed to the intense agricultural development and the rapid human population growth around the plantations (Hendrickx and Napala, 1997). In general, most of the flies in this group are less suited to desiccating conditions, and therefore survive in thick riverine forests with enough shelter from winds and heat. This is especially the case for the three fuscipes subspecies which are confined to hygrophytic habitats, rarely far from open water lacustrine or riverine habitats. Glossina tachinoides, although typically a riverine species, were found in northern Nigeria to extend into human-inhabited savanna woodlands during the wet season, also displaying strong adaptations to peridomestic habitats (Kuzoe et al., 1985).
With the exception of Glossina brevipalpis and Glossina longipennis, all the tsetse flies in the fusca group are found in West African forests. None of the species in the fusca group are vectors of human trypanosomiasis, however both Glossina fusca and Glossina medicorum are efficient vectors of trypanosomes to livestock (mainly Trypanosoma vivax), causing considerable economic burden. Distributions of the fusca group tsetse depend primarily on forest vegetation and climatic factors. With the exception of G. longipennis, most fusca group species inhabit moist, evergreen habitats either in riverine forests within savannas (such as Glossina medicorum) or in dense and wet rain forests (Glossina tabaniformis and Glossina nigrofusca). In stark contrast to the rest, the G. longipennis species lives in one of the driest habitats inhabited by tsetse flies. Due to its pupal adaptation to dry conditions, its primary habitat - consisting of dry deciduous acacia bush – are discontinuously spread throughout East Africa (Glasgow, 1963).
The intrinsic vectorial capacity of a tsetse fly refers to the intrinsic capability of a fly to develop a metacyclic infection (Le Ray, 1989). In general, infection rates of the three salivarian trypanosome subgenera (Dutonella, Nannomonas, and Trypanozoon) are usually low in populations of tsetse, with infections rates determined by the parasite, the host, the vector, and the environment (Jordan, 1974). Trypanosoma infection rates in tsetse flies vary greatly from species to species, with T. vivax ranking the highest and T. brucei species ranking the lowest.
Factors Influencing Trypanosome Infection Rates in Tsetse
Tsetse fly species differ in their ability to develop infections, as discussed earlier. Female tsetse flies usually have higher infection rates than males, partially because females live longer than males and therefore have a higher probability of infection. However is has not yet been determined whether the sex of a fly influences the infection rate (Kazadi 1991, Mihok, 1992). Within species it is found that infection rates vary greatly depending on individual host factors. It has also been shown that susceptibility of flies to infection with T. brucei is also due to a maternally inherited characteristic, associated with the presence of intracellular rickettsia-like organisms (RLOs). Tsetse flies carrying RLOs in the midgut were found to be six times more likely to be infected with trypanosomes than those without (Maudlin et al., 1990). Within any given species, individuals and sexes of the same species respond differently to infection suggesting some involvement of the genetic differences (Jordan, 1974).
The mating behavior of tsetse flies have received much attention because of the development of the Sterile Insect Technique (SIT) for tsetse fly control. The existence of tsetse flies at low densities in certain areas (as low as 40 per km2) suggest highly specific mating mechanisms involving visual and olfactory responses (Glasgow, 1963). Female tsetse flies only need to mate once in their lifetime, but multiple matings have been known to occur occasionally (Jaenson, 1980). Cross-mating is possible in areas where habitats of different species overlap, however male hybrids are infertile (Jackson, 1950). Mating of female is mostly confined to early life with mean duration of mating declining by age. Most female flies are successfully inseminated even at very low population densities, usually during their first blood meal right after emergence from the pupal stage (Teesdale, 1940).
Tsetse flies use visual and olfactory characteristics to recognize potential hosts before initiating host-oriented responses. There are a series of behavioral responses involved in the process of obtaining a bloodmeal. Host-seeking behaviors are influenced by endogenous and exogenous factors. Endogenous factors include circadian rhythm of activity level of starvation, age, sex and pregnancy status of the fly (Brady, 1972). Exogenous factors include temperature, vapor pressure, visual and olfactory stimuli, and mechanical stimulation (Huyton and Brady, 1975). There are four stages of host-locating behavior as described by Wilemse and Takken (1994):
Generally, the tsetse fly will detect an odour plume upwind until it visually recognizes the host. After landing on the host, heat stimulation cause a probing and feeding response. It was found that feeding activity for the morsitans group tsetse were highest during the early morning and the late afternoon due a combination of both external temperatures (20%) and circadian rhythm (80%) (Brady and Crump, 1978).
It is thought that tsetse flies originally fed on reptiles living in forests and later became adapted to feeding on mammals. Adaptations to feeding on warthogs are thought to be one of the pathways by which tsetse flies entered the savanna ecosystems, subsequently evolving as the separate morsitans subgenus (Ford, 1970). Most of our knowledge about species specific host preferences is derived from blood meal analyses of captured flies.
Summary of main hosts of tsetse determined from analysis of bloodmeals (adapted from Moloo, 1993)
Unlike most insect species (r-strategists) that produce large quantities of eggs, fertilized female tsetse flies (k-strategists) “give birth” to one larva. A typical female tsetse fly will produce one full grown larva approximately every 9-10 days depending on temperature and humidity. A single egg will hatch and develop to a third-stage larva in the uterus of the female fly, where it is nurtured and supplied with nutrients. This reproductive process is known as adenotrophic viviparity . This form of reproduction ensures the higher degree of survival of each offspring, but is also the reason why reproductive rates are considerably low in tsetse fly populations. In laboratory colonies, a single adult female can produce up to 12 offspring this way, however in the wild the number is speculated to be lower (Leak, 1999). Larviposition takes place when the third-instar larva is deposited onto a suitable site, usually soil or sand depending on the species, and the larva burrows down to its optimum depth to become a pupa. Usually an adult fly will emerge after the puparial period which varies according to temperature but on average is around 30 days at 24°C (Leak, 1999). Longevity of the adult fly varies greatly according to seasonal factors. For the general tsetse population to increase, it is critical that the average female lifespan exceed 36 days. During optimal conditions, female flies can live as long as 3 months, producing as much 10 offspring during her lifetime (Jordan, 1986).
Many population control methods in the past have been successful because tsetse populations are far more vulnerable to disruptions in life cycles than insects that are r-strategists, such as mosquitoes. When both larval and adult mortality rates are artificially increased through control methods such as fly traps, insecticide spraying, and sterile insect techniques, the reduction in reproductive rate is profound.
Although epidemics as large as the ones in Uganda at the turn of the century have not been repeated, there is much concern over the re-emergence and increase in the number of sleeping sickness cases being reported every year in Africa. In 1994, there were an estimated 150,000 cases in Congo, with prevalences as high as 70% in some villages (Cattand, 1994). Despite the WHO projection of 60 million people at risk in Africa, only a fraction of the population at risk is currently under surveillance, and relatively few cases are accurately diagnosed annually (Knudsen and Slooff, 1992). Although sleeping sickness was largely under control during the 1960s, recent epidemics have been strongly associated with political and civil unrest in West and Central Africa resulting in mass movement of populations into areas formerly uninhabited by humans.
West African sleeping sickness is typically a chronic disease, making it a difficult disease to diagnose in the field. Low levels of trypanosomes in circulating blood make it difficult to detect the presence of parasites in blood smears, requiring more sophisticated means of detecting trypanosomes such as with the use of miniature anion-exchange / centrifugation (mAEC) technique. In comparison to the East African form, T. b. gambiense has a longer evolutionary history with humans, having successfully adapted to establishing infections in human hosts without manifesting severe symptoms. Astonishingly, infection rates of T. b. gambiense in wild glossina populations are as low 0.1%, even in areas with an epidemic of sleeping sickness (Jordan, 1986).
Vectors of the West African sleeping sickness are species of the palpalis group, most of which are in close contact with humans. Several different reservoirs for T. b. gambiense have been identified, strongly suggesting that the persistence of sleeping sickness in human populations may be maintained by other animals, such as the African domestic pig (Watson, 1962; Gibson et al., 1978;, Mehlitz et al., 1982). However, T. b. gambiense has not been observed or proven experimentally to reach significantly infectious levels of parasitemia in other reservoir hosts. Although it is widely accepted that the human-to-fly contact is the main route of transmission, some suggest a minor cycle involving an animal reservoir may help explain the re-emergence and persistence of the disease in West Africa (Noireau et al,. 1989).
The epidemiology of T. b. gambiense sleeping sickness is far from being fully understood. Despite the low levels of parasitemia in humans, the disease has successfully established endemicity in many regions of West Africa . It has also long been observed that the incidence of disease is not related to the density of the glossina populations, and that epidemics often occur in areas where the density of the vector is low (Jordan, 1986). In Nigeria, sleeping sickness occured in the north where the distribution of G. p. palpalis and G. tachinoides were scarce and restricted to vegetation close to watercourses during the dry season (Edeghere et al., 1989). In Southern Nigeria, the same species of tsetse flies are found in abundance due to favorable climatic conditions, yet cases of sleeping sickness have never been observed. It is thought that the nature of the human-fly contact is of particular importance in the transmission of T. b. gambiense and the distribution of the disease, and that human-fly contacts can be classified as “personal” or “impersonal” depending on the ecological circumstances of the interaction (Nash and Page, 1953). “Personal” contact refers to situations where fly movements are restricted to areas where exposure to humans are frequent, such as a watering hole or a stream, and single tsetse fly can have multiple opportunities to feed on humans. “Impersonal” contact occurs when fly movements are less restricted, and where repeated contacts are not likely. In general, ecological isolation of tsetse flies in the vicinity of human populations lead to increased “personal” contact. Climatic stress, lack of natural hosts where humans have destroyed wild animals close to villages, or clearing vegetation for cultivation are all examples of restrict movements of palpalis group vectors.
East African sleeping sickness differs from West African sleeping sickness in both its epidemiology as well as its clinical manifestations in mammalian hosts (Baker, 1974). The clinical symptoms of East African sleeping sickness are more severe, and the onset of the disease is rapid. In contrast to T. b. gambiense, T. b. rhodensiense occurs with higher levels of parasitemia in ungulates, and humans are the adventitious hosts. The vectors of T. b. rhodensiense are the G. mositans subspecies, G. pallidipes and G. swnnertoni species from the morsitans group, and on lesser occasions the peridomestic vectors from the palpalis group, G. fuscipes and G. tachinoides . Sporadic cases usually arise from among those in the population whose activities bring them into contact with the savannah woodland habitats of the morsitans group. Although the vectors normally feed on game animals, under extreme situations where “personal” contact is increased due to social and/or environmental factors, a human-fly-human transmission cycle may ensue resulting in an outbreak. Droughts and political turmoil are known to increase the number of cases when entire communities relocate to hitherto unoccupied areas in search of safety or fertile lands and water (Molyneaux and Ashford, 1983).
The clinical manifestations of both forms of sleeping sickness are usually quite different, but can be easily confused because of the variability of symptoms and length of time until onset depends heavily on host characteristics (Molyneaux and Ashford, 1983). The chancre, a leathery swelling at the site of the bite, is usually the first symptom of the disease, primarily for T. b. rhodensiense. Within weeks, those with opportunistic levels of infection with T. b. rhodensiense start to experience irregular intermittent fevers associated with the waves of parasitaemia that are characteristic of T. b. rhodensiense infections. For T. b. gambiense, lymphoadenopathy occurs more frequently. Oedema of the face is another frequent sign of infection, and anemia may be present, particularly in T. b. rhodensiense.
There are two stages to sleeping sickness; the early stage refers to the hemolymphatic infection, and the late stage refers to infection of the CNS. The development of late stage sleeping sickness may not occur for decades in West African sleeping sickness, and a patient may only suffer mildly from fatigue due to the occasional rises of parasites in the blood. However, East African sleeping sickness is far more virulent, and can develop into late stage sleeping sickness within weeks. Although symptoms and signs associated with nervous system involvement are varied for both East and West African sleeping sickness, advanced disease epileptic attacks, maniacal behavior, somnolence and coma are some typical late stage symptoms (Dumas and Bisser, 1999). Both treatment options and survival rates are drastically reduced once the trypanosomes infect the CNS.
Today, there are only a handful of active drugs available for treatment of human African trypanosomiasis. No significant development has been made over the last 2 decades. The current line of treatment is problematic for many reasons: firstly, the drugs are harmfully toxic requiring extensive hospitalization. Secondly, regular follow-ups to check for relapse is essential but difficult in many of the areas where sleeping sickness is endemic.
Summary of drugs available for treatment of human African trypanosomiasis
Treatment of the hemolymphatic stage is based on pentamidine and suramine . Melarsoprol, an arsenic compound, is the only treatment option available for late stage sleeping sickness because of its ability to penetrate the blood-brain barrier. Unfortunately, even when administered under careful medical attention, the treatment has a mortality rate as high as 12 % (Apted, 1957). Eflornithine is effective against both stages of T.b gambiense infection, but not against T. b rhodensiense (Iten et al., 1995). Although the most recent and effective drug against sleeping sickness, it is not widely available, difficult to administer, and costly for use under African health care conditions (Bouteille et al., 2003) So far, only 2000 patients in therapeutic trials have been treated with eflornithine.
Use of pentamidine as a form of mass chemoprophylaxis has proven to be an effective form of prevention and control in endemic foci of T. b. gambiense.
In the absence of a vaccine for trypanosomosis and with the looming threat of further trypanocidal drug resistance, the most theoretically desirable means of controlling the disease is through controlling the vector population (Leak, 1999). Although complete eradication of the vector is impossible, the most successful attempts at controlling tsetse flies are likely to be at the extreme limits for survival of the fly, where both the density of the fly is low and “personal” contact with humans may be highest (Rogers, 1979).
There are several different control techniques available today, but the use of chemicals in controlling tsetse populations is still the most common method. In brief, whether aerial or from the ground, residual insecticides such as organochlorines (DDT, Dieldrin, Endosulfan), pyrethroids (deltamethrin, permethrin, and alphamethrin), and avermectins (ivermectin) are used to target areas where human-to-fly contact are likely. Pyrethroids are preferred because they are rapidly degraded in soil and are environmentally safe, unlike organochlorines, carbamates and organophosphates that bioaccumulate in the food chain and are highly toxic to mammals and other vertebrates. Despite being effective, the use of organochlorines and organophosphates are now banned for widespread outdoor spraying. Susceptibility to insecticides varies from one species to another, and between the different classes of species (Leak, 1999). The most common form of administering insecticides is through the use of pressurized knapsacks. Over 200,000 km2 of tsetse-infested land has been cleared by ground-spraying in West Africa, mainly in Nigeria, and proved to be successful and cost-effective (Barrett, 1997). Although the process is highly labor intensive and limited in geographical scope, the spraying is administered discriminatively to day and night resting sites during the dry season and are much more effective than indiscriminate spraying from the air or from vehicles.
Traps and targets are mechanical devices used to kill or weaken tsetse flies through insecticides or various trapping methods. The use of traps and targets to control tsetse populations have been successful primarily because tsetse flies are k-strategists with a low rate of reproduction, and require very little sustained mortality pressure to bring about a reduction in population or even eradication from an area (Weidhaas and Haile, 1978). Haargrove (1988) estimated that an additional mortality of 4% per day imposed on female flies was enough to cause extinction, in the absence of immigration. The traps and targets attract tsetse flies by taking advantage of their primary host-seeking behaviors, visual and olfactory stimulation. The developments of potent attractants in the last 20 years as well as the production of second-generation synthetic pyrethroid insecticides are making this form of control technique highly successful (Wall and Langely, 1991).
There are many prototypes of traps and targets customized to attract as many tsetse flies as possible in different environments, with a strong emphasis on designs that are easy to duplicate and maintain locally. Although most traps are strongly reliant on chemical attractants and insecticides, some have recently been designed to attract tsetse flies based on visual stimulation alone and to kill tsetse flies through a trapping mechanism (NGU and NG2B traps). Although these traps may not be as efficient in attracting and killing tsetse flies, they are far more affordable and feasible to implement in resource poor settings. Such traps were used to successfully suppress the tsetse fly populations in Nguruman, Kenya . With the Maasai community involved, 190 homemade NG2B traps were deployed over 100 km2, and a 98-99% G. pallidipes reduction was achieved over a 10 month period, and the reinvasion was kept relatively low during the rainy season (Dransfield et al, 1990). Targets and traps are usually deployed in and around areas where human-fly contacts are greatest, such as streams frequented by villagers, or fringes of cultivated fields. All aspects of these targets and traps, from its design and color to their strategic placement, are reliant on understanding the biology and behavioral ecology of the various tsetse fly species.
Exploiting the knowledge that tsetse flies concentrated in certain areas lead to numerous bush-clearing projects all over West and East Africa to drastically alter and maintain the area unsuitable for tsetse fly habitation. Discriminative bush-clearing was used in Uganda to control for G.m. centralis by clearing taller Acacia trees in the Ankole district (Harley and Pilson, 1961). In Tanzania, between 1923 and 1930, bush-clearing methods were also widely employed to stop the spread of sleeping sickness epidemic in Maswa district, where G. swynnertoni was prevalent (Leak, 1999). Similar tactics were used in Ghana to control sleeping sickness around villages were human-fly contacts were high (Morris, 1949). Despite the apparent success of these methods, it is widely accepted that bush-clearing is unsuitable as a long term control measure due to the expense and speed of reinvasion, as well as the environmental damage it causes through soil erosion, decreased soil fertility, and its adverse effects on water supplies.
One of the more modern methods of non-insecticidal control is the Sterile Insect Technique (SIT) which was first considered as a means to control tsetse by Simpson in 1958. This technique relies on the mating of wild females with sterile male flies. Physiologically, female tsetse flies are only required to mate once to store sperm in its spermathecae in sufficient quantity such that fertilization can occur over its entire reproductive life. Mating with a sterile male would thus result in no offspring. However, SIT was considered to be impractical for control of high-density tsetse populations above 1000 males per square mile due to the large number of sterilized males that would be required. For SIT to be effective, it has been estimated that 10% of the females in the population need to be inseminated, and in order to achieve that, the number of sterile males released must constitute 80% of the male population (Rogers and Randolph, 1985).
Sterilization of male tsetse flies can be carried out by
In 1994, an eradication program conducted in Zanzibar by the authorities and the International Atomic Energy Agency (IAEA) used a combination of insecticide-impregnated traps and SIT to completely eradicate the entire tsetse population of Zanzibar by 1996 (IAEA, 1997). Over 7.8 million gamma irradiated sterile male G. austeni flies were released over the island with a ratio of 50:1 sterilized males to wild males. This campaign may have been successful in part because there is virtually no immigration of tsetse flies into the island.
After the publication of works such as “Silent Spring” (Carson, 1962), public awareness of the dangers associated with insecticides are increasingly changing the way we treat our environment, and the way we institute environmental controls. Consequently, efforts to introduce more environmentally friendly methods of vector control, such as the use of traps without insecticides, challenges us to understand more about the vectors that transmit the disease, as well as the ecological balance that we - as humans - strike with them.
We live in a world where various technical means of control are available to address the spread of the disease. However, sleeping sickness is a disease of the developing world, where despite the multitude of control strategies, the issues have widely been neglected and abandoned. One of the key components required to bring about effective change is to consider the sustainability of the control strategy, and to encourage local communities to take ownership over the process, thereby empowering people to take an active role in an environmentally conscious solution. Increasing knowledge through culturally sensitive education, providing technical support, and a long-term commitment of basic resources to beneficiary communities is essential for large-scale tsetse control (Swynnerton, 1925).
Alongside efforts to reduce the spread of disease through environmental controls, there is also an urgent need to improve current surveillance and diagnostic procedures. Mortality can be drastically reduced when cases can be diagnosed early enough to prevent the progression of late-stage sleeping sickness. Training and resources are desperately needed in endemic areas for proper diagnostics and sero-surveillance.
Perhaps the most mysterious aspect of this disease relates to the issue of treatment options, and the availability of drugs in Africa . Drug and vaccine development for diseases in developing countries have always been lagging, and unfortunately, trypanocidal drugs are no exception. An estimated 300,000 – 500,000 people are currently infected and suffering from the disease with no hope for treatment. In 2000, the USFDA approved the use of eflornithine by the Bristol-Myers Squibb Company and The Gillette Company in a product called Vaniqa TM, a topical eflornithine HCl cream to remove facial hair. Perhaps some of the profits generated from the sale of this form of the drug will be used to underwrite the free use of the drug in Africa, similar to what has already happened at Merck, who donates invermectin for the treatment of river blindness, and at Pfizer Inc for their azithormycin give away program for the treatment of trachoma.
Atkins J. (1978) Sleeping Sickness. Tropical Medicine and Parasitology, Classic Investigations. Cornell University Press, Ithaca, New York, 181-182
Baker J.R. (1974) Epidemiology of African sleeping sickness. Trypanosomiasis and Leishmaniasis with Special Reference to Chagas Disease. CIBA Foundation Symposium, 29-50.
Baldry D.A.T. (1980) Loca distribution and ecology of Glossina palpalis and G. tachinoides in forest foci of west African human trypanosomiasis, with special reference to associations between peri-domestic tsetse and their hosts. Insect Science and its Application, 1, 85-93.
Barret J.C. (1997) Control strategies for African trypanosomiases: their sustainability and effectiveness. Trypanosomiasis and Leishmaniasis. CAB International, Wallingford, 347-362.
Bouteille B. et al. (2003) Treatment perspective for human African trypanosomiasis. Fundamental & Clinical Pharmacology, 17, 171-181.
Brady J. (1972) The visual responsiveness of the tsetse fly Glossina morsitans Westwood (Glossinidae) to moving objects: the effects of hunger, sex, host odor and stimulus characteristics. Bulletin of Entomological Research, 62, 257-279.
Brady J., Crump A.J. (1978) The control of circadian activity rhythms in tsetse flies: environment or physiological clock? Physiological Entomology, 3, 177-190.
Cattand P. (1994) World Health Organization Press Release. WHO/73, 7 October 1994.
Dransfield R.D. et al. (1990) Control of tsetse fly (DipteraL Glossinidae) populations using traps at Nguruman, south-west Kenya . Bulletin of Entomological Research, 80, 265-276.
Dumas M., Bisser S. (1999) Clinical aspects of human African trypanosomiasis (Ch. 13), Progress in human African rypanosomiasis, sleeping sickness . Springer-Verlag, Paris, 215-233.
Edeghere H. et al. (1989) Human African trypanosomiasis (sleeping sickness): new endemic foci in Bendel state, Nigeria . Tropical Medicine and Parasitology, 40, 16-20.
Ford J. (1960) The influence of tsetse flies on the distribution of African cattle. Proceedings of the 1 st Federal Scientific Congress, Salisbury, 357-365
Gibson W.C. et al. (1978) The identification of Trypanosoma brucei gambiense in Liberian pigs and dogs by isoenzymes and by resistance to human plasma. Tropenmedizin und Parasitologie, 29, 335-345.
Glasgow J.P. (1963) The distribution and abundance of tsetse. International Series of Monographs on Pure and Applied Bioology, 20, Pergamon Press, Oxford, 241.
Hargrove J.W. (1981) Tsetse: the limits to population growth. Medical and Veterinary Entomology, 2, 203-217.
Harley J.M.B., Pilson R.D. (1961) An experiment in the use of discriminative clearing for the control of Glossina morsitans westwood in Ankole, Uganda . Bulletin of Entomological Research, 52, 561-576.
Hendrickx G., Napala, A. (1997) Le contròle de la trypanosome ‘á la carte': une approche intègrèe basèe sur un Système d'Information Gèographique. Acadèmie Royale des Sciences d'Outhre Mer. Mèmoires Classe Sciences Naturelles and Mèdicales. In 8 th Nouvelle Sèrie, Tome24-2, Bruxelles, 1997, 100.
Jackson C.H.N. (1950) Pairing of Glossina morsitans Westwood with G. swynnertoni Austen (Diptera). Proceedings of the Royal Entomological Society of London (A), 25, 106.
Jaenson T.G.T. (1980) Mating behavior of females of Glossina pallidipes. Bulletin of Entomological Research, 70, 49-60.
Jahnke H.E. (1982) Livestock Production Systems in Livestock Development in Tropical Africa . Kieler Wissenschaftsverlag Vauk, Kiel, FRG.
Jordan A.M. (1974) Recent developments in the ecology and methods of control of tsetse flies (Glossina spp.) (Diptera, Glossinidae) – a review. Bulletin of Entomological Research, 63, 361-399.
Jordan A.M. (1976) Tsetse flies as vectors of trypanosomes. Veterinary Parsitology, 2, 143-152.
Jordan A.M. (1986) Trypanosomiasis Control and African Rural Development. Longman, London .
Kazadi J.M.L. et al., (1991) Etude de la capacitè vectorielle de Glossina palpalis gambiensis (Bobo-Dioulasso) vis-á-vis de Trypanosoma brucei brucei EATRO 1125. Revue d'Élevage et de Mèdecine Vètèrinaire des Pays Tropicaux, 44, 437-442.
Knudsen A.B., Slooff R. (1992) Vector-borne disease problems in rapid urbanization: New approaches to vector control. Bulletin of the World Health Organization, 70, 1-6.
Kuzoe F.A.S et al. (1985) Observations on an apparent population extension of Glossina tachinoides Westwood in southern Ivory Coast . Insect Science and its Application, 6, 55-58.
Leak S.G.A. (1999) Tsetse Biology and Ecology, CABI publishing, New York .
Le Ray D. (1989) Vector susceptibility to African trypanosomes. Annales de la Société Belge de Médecine Tropicale, 69, Supplément 1, 167-171
Maudlin I. et al. (1990) The relationship between Rickettsia-like organisms and trypanosome infections in natural populations of tsetse in Liberia . Tropical Medicine and Parasitology, 421, 265-267.
McKelvey J. J. (1973) Man Against Tsetse: Struggle for Africa . Cornell University Press, Ithaca and London .
Mehlitz D. et al. (1982) Epidemiological studies on the animal reservoir of Gambiense sleeping sickness. Part III. Characterization of Trypanozoon stocks by isoenzymes and sensitivity to human serum. Tropenmedizin und Parasitologie, 33, 113-118.
Mihok S. et al. Trypanosome infection rates in tsetse flies (Diptera: Glossinidae) and cattle during control operations in the Kagera river in Rwanda . Bulletin of Entomological Research. 82, 361-367.
Moloo S.K. (1993) The distribution of Glossina species in Africa nd their natural hosts. Insect Science and its Application, 14, 511-527.
Molyneaux D.H. (1980) Host-trypanosome interactions in Glossina. Insect Science and its Application, 1, 39-46.
Molyneux D.H., Ashford R.W. (1983) The Biology of Trypanosoma and Leishmania, Parasite of Man and Domestic Animals. Taylor and Francis, London .
Morris K.R.S. (1949) Planning the control of sleeping sickness. Transactions of the Royal Society of Tropical Medicine and Hygiene, 43, 165-198.
Nash T.A.M., Page W.A. (1953) On the ecology of Glossina palpalis in northern Nigeria . Transactions of the Royal Entomological Society of London, 104, 71-170.
Noireau F. et al. (1989) The epidemiological importance of the animal reservoir of Trypanosoma brucei gambiense in the Congo . Tropical Medicine and Parasitology, 40, 9-11.
Rogers D.J. (1979) Tsetse population dynamics and distribution. A new analytical approach. Journal of Animal Ecology, 48, 825-849.
Rogers D.J., Randolph S.E. (1985) Population ecology of tsetse. Annual Review of Entomology, 30, 197-216.
Swynnerton C.F.M. (1925) The tsetse fly problem in the Nzega sub-district Tanganyika Territory . Bulletin of Entomological Research, 16, 99-110.
Teesdale C. (1940) Fertilization in the tsetse fly, Glossina palpalis, in a population of low density. Journal of Animal Ecology, 9, 24-26.
Wall R., Langley P.A. (1991) From behavior to control: The development of traps and target techniques for tsetse fly population management. Agricultural Zoology Reviews, 4, 137-159.
Watson H.J.C. (1962) The domestic pig as a reservoir of Trypanosoma gambiense. ISCTR 9 th meeting, Conakry, 327.
Weidhaas D.E., Haile D.G. (1978) The domestic pig as a reservoir of Trypanosoma gambiense. ISTR 9 th meetings. Conakry, 327.
WHO (1995) Planning Overview of Tropical Diseases Control. Division of Tropical Diseases. World Health Organization, Geneva .
Williamse L.P.M, Takken W. (1994) Odor-inducedhost location in tsetse flies (Diptera: Glossinidae). Journal of Medical Entomology, 31, 774-794